Characterization of a culture-derived CD15+ CD11b- promyelocytic population from CD34+ peripheral blood cells
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چکیده
منابع مشابه
Gene Expression Profile of Adherent Cells Derived From Human Peripheral Blood: Evidence of Mesenchymal Stem Cells
Mesenchymal stem cells (MSCs) provide a novel option in cellular therapy and tissue engineering. Recent studies indicated that it is possible to obtain MSCs from peripheral blood by attachment ability to plastic surface. To evaluate adherent cells derived from peripheral blood, their expression profile and surface markers were investigated. The results of RT-PCR indicated that these cells expre...
متن کاملExpression of the nlsLacz gene in dendritic cells derived from retrovirally transduced peripheral blood CD34+ cells.
BACKGROUND AND OBJECTIVE Gene transfer and expression of exogenous genetic information coding for an immunogenic protein in antigen presenting cells (APCs) can promote an immune response. This was investigated by retroviral transfer of a marker gene into CD34+ derived APCs. DESIGN AND METHODS To achieve long term expression of a specific transgene in APCs, G-CSF mobilized peripheral blood CD3...
متن کاملPeripheral Blood-Derived Mesenchymal Stem Cells: Growth Factor-Free Isolation, Molecular Characterization and Differentiation
Background and Objectives: The mesenchymal stem cells derived from peripheral blood (PB) have been recognized as a promising source for allogeneic cell therapy. The aim of this study was to investigate the isolation, growth and differentiation ability of peripheral blood-isolated mesenchymal stem cells. Methods: The mononuclear cells were purified from fr...
متن کاملFunctional differences between dendritic cells derived from CD34+ bone marrow and peripheral blood stem cells.
BACKGROUND AND OBJECTIVE It has been previously demonstrated that dendritic cells (DCs) are characterized by an immature stage with high antigen internalization capacity, followed by a mature stage with predominantly immunostimulatory ability. The shift from the immature to the mature state can be induced in vitro by the addition of tumor necrosis factor-a (TNFa). The aim of our study was to in...
متن کاملCulture and Expansion of Rat Mesenchymal Stem Cells Using the Serum Prepared From Rat’S Peripheral Blood
Purpose: The aim of this study is to evaluate the effects of prepared serum from rat peripheral blood on growth and viability of rat bone marrow-derived mesenchymal stem cells (MSCs) in vitro. Materials and Methods: Bone marrow cells prepared from rat’s tibia and femur central canal were cultured either in medium containing fetal bovin serum (FBS) or rat peripheral blood-derived serum (PBDS) f...
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ژورنال
عنوان ژورنال: Journal of Leukocyte Biology
سال: 1997
ISSN: 0741-5400
DOI: 10.1002/jlb.62.4.480